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AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) is a ribonucleoside analog of AMP that activates AMP-activated protein kinase (AMPK). With molecular formula C₉H₁₅N₄O₈P, it serves as a key tool for studying cellular energy regulation and AMPK-mediated metabolic signaling pathways.
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AICAR is widely utilized in metabolic research as a potent activator of AMPK, mimicking the effects of cellular AMP to regulate glucose uptake and fatty acid oxidation. This property makes it essential in exploring pathways governing mitochondrial biogenesis, ATP generation, and metabolic stress responses [1].
Researchers use AICAR to examine how energy-sensing enzymes influence osteoblast differentiation and mineralization. In bone and muscle models, it helps delineate the role of AMPK activation in skeletal tissue regeneration and differentiation dynamics [1].
AICAR has been used in neuroglial and macrophage cell studies to analyze how AMPK activation suppresses pro-inflammatory mediators like iNOS and TNF-α under oxidative or metabolic stress. This application supports its use in mapping cellular energy-immune interplay [1].
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